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Greg O
Posted on Friday, March 03, 2006 - 10:10 am:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

MBD is:

7-(P-Methoxybenzlamino-4Notrobenz-2-Oxa-1,3-Diazile). A yellow dye that will fluoresce when exposed to selected wavelengths of light; Used to visualize cyanoacrylate fumed friction ridge detail.
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Seth
Posted on Wednesday, February 22, 2006 - 05:39 pm:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

A magnetic stirrer is a common piece of lab equipment and shouldn't interfere with any kind of chemical processes. Like Kathy said- it makes things alot easier. Your school may have some articles of the JFI (thats the Journal of Forensic Identification) and you might look through those for articles on MBD(methoxybenzlamino-something) or find out who your school bought it from and write to the vendor. It is a pretty old reagent so there is probably something out there on it. Good luck!
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Connie Muller
Posted on Friday, February 27, 2004 - 02:40 pm:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

Thanks everyone for their responses to my question. Of course, now I have another MBD related query. Has anyone performed any experiments with MBD and its possible interference with subsequent DNA results? Most of the studies I can find have to do with rhodamine or some of the other more popular dye stains.
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Kathy Saviers
Posted on Friday, February 20, 2004 - 11:51 am:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

You can go to the FBI LP Processing guide through this web site (get out of the Forum and go to the main page, go to the expert area and scroll down until you find it.

There is another fabulous web site done by the Cheasapeake Bay Division of the IAI. Here is the link to the particular page on MBD. But, don't stop there, check out all of the reagents and dyes discussed.

www.cbdiai.org/Reagents/mbd.html
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David Fairhurst
Posted on Thursday, February 19, 2004 - 08:04 am:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

Oh!
Then this is a treatment that I am not aware of.
Could you let me know what it is and when you use it please?

David
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Kathy Saviers
Posted on Wednesday, February 18, 2004 - 06:25 pm:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

MBD is NOT molybdenum disulphide and it is NOT magnetic.

The purpose of a magnetic stirrer is like an electric mixer in the kitchen. It stirs the stuff so you don't have to. The device looks like a tall hot plate. There is a motor inside with a magnet attached to it. You place your beaker with the solution on top and drop another magnet in the beaker. The two magnets "grab hold" of each other and when the motor turns the magnet on the inside of the box, the magnet in the beaker also turns, mixing the solution.

Sometimes the instructions may state to stir the solution for 30 minutes, or until the powder (or liquid) is thoroughly mixed. If I can walk away and do other work while the solution is mixing, that is a good thing.
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Michele
Posted on Monday, February 16, 2004 - 10:55 am:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

It does say that a magnetic stirrer is needed in the "equipment needed" section. I think the magnetic stirrer is need to make the stock solution, but when you're making the working solution you don't need it because you're mixing all liquids. I think you 'can' use a magnetic stirrer, but it really isn't needed.

Michele
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David Fairhurst
Posted on Monday, February 16, 2004 - 04:45 am:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

Maybe MBD (molybdenum disulphide?) is magnetic.
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Connie
Posted on Saturday, February 14, 2004 - 12:46 pm:   Edit Post Delete Post View Post/Check IP Print Post    Move Post (Moderator/Admin Only) Ban Poster IP (Moderator/Admin only)

Hello,
While doing some research on the internet, I came across the FBI's processing guide. I pulled up their info on mixing MBD and noticed that under the instructions for mixing the working solution, they state that a magnetic stirrer should not be used. Any thoughts on why this would be?

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